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  • M217 - Monoclonal Anti-Nicotinic Acetylcholine Receptor (α1, α3, α5 Subunits) antibody produced in rat

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M217 Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α1, α3, α5 Subunits) antibody produced in rat

clone mAb 35, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Ion Channels,
conjugate   unconjugated
clone   mAb 35, monoclonal
biological source   rat
application(s)   immunohistochemistry (frozen sections): 1:3,000
species reactivity   eel, chicken, human
should not react with   Xenopus
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   CHRNA1
Gene Information   human ... CHRNA1(1134), CHRNA3(1136), CHRNA5(1138)

Description

Specificity

Binds to the main immunogenic region on the extracellular surface of the α1 subunit of muscle AChR′s and to homologous regions on α3 and α5 subunits of neuronal AChR′s. The epitope is located on the extracellular surface of the α1 subunit of the muscle nicotinic acetylcholine receptor. The antibody binds to denatured chick α5 AChR, but does not bind well to denatured α3, α4, α7, α8, and β2. It may also bind β3 due to homology of main immunogenic region. May be used to localize and detect the α1, α3 and α5 subunits of the nicotinic acetylcholine receptor (AChR).

Immunogen

nicotinic acetylcholine receptor from the electric eel Electrophorus.

Physical form

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05 % sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sold with permission of the Salk Institute

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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