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M2272 Sigma-Aldrich

Melittin from honey bee venom

≥85% (HPLC)

  • CAS Number 20449-79-0

  • Empirical Formula (Hill Notation) C131H229N39O31

  • Molecular Weight 2846.46

  •  MDL number MFCD00076868

  •  PubChem Substance ID 24896718

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Properties

Related Categories Antibiotics, Biochemicals and Reagents, Calcium Antagonists, Cell Biology, Cell Signaling and Neuroscience,
InChI Key   VDXZNPDIRNWWCW-JFTDCZMZSA-N
assay   ≥85% (HPLC)
Mode of action   cell membrane | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
foreign activity   Phospholipase A2 ≤0.5%
storage temp.   −20°C

Description

Amino Acid Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2

General description

Chemical structure: peptide

Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct haemolytic agent.† The encoded protein containins 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840 Daltons and tetrameric form has molecular weight of approximately 12,500 Daltons.

Application

Melittin from honey bee venom has been used:
• In 3-(4, 5-dimethyl thiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay to determine its cytotoxicity effect on the growth of human cell lines.
• To study the anti-microbial activity of melittin on the growth of Borrelia burgdorferi in in vitro conditions.
• As a positive control in hemolysis assay and as a cytotoxic agent against HeLa cells.

Packaging

1, 5, 25 mg in glass bottle

Biochem/physiol Actions

Melittin acts as an anti-coagulating protein by increasing the time of blood clotting in vitro. Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment.

Binds calmodulin in a Ca2+-dependent manner; inhibits Na+-K+-ATPase.

Other Notes

The principle hemolytic component of honeybee venom.

Safety & Documentation

Safety Information

RIDADR 
UN 3462 6.1 / PGI
WGK Germany 
3
RTECS 
OS3960000

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antimicrobial Peptides

With bacterial resistance and emerging infectious diseases becoming potential threats to humans, ribosomally synthesized antimicrobial peptides have become a promising focus area in antibiotic resear...
Chloe McClanahan
BioFiles 2009, 4.3, 4.
Keywords: Antibiotics, Antimicrobials, Antiparasitics, Antivirals, Apoptosis, Cancer, Clinical, Diseases, Environmental, Fermentation, Food Safety, Gene expression, Genetic, Genetics, Infectious Diseases, Microbiology, Peptide synthesis, Pesticides, Respiratory

Peer-Reviewed Papers
15

References

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