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M2420 Sigma-Aldrich

Monoclonal Anti-Monocyte Chemotactic Protein-1 antibody produced in mouse

clone 24822, purified immunoglobulin, lyophilized powder

Synonym: Anti-CCL2, Anti-MCP-1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   24822, monoclonal
biological source   mouse
application(s)   neutralization: suitable
  western blot: suitable
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P13500
Gene Information   human ... CCL2(6347)

Description

General description

Monocyte Chemotactic Protein-1 (MCP-1) is a chemotactic factor that belongs to CC chemokine family and can attract basophils and monocytes but not eosinophils or neutrophils. It is produced in keratinocytes, fibroblasts, hepatoma cells and Type II pneumocytes in response to IL-1. It has a crucial role in recruiting immune cells to the site of antigens as well as behaves as an inflammatory mediator which stimulates the directional migration of T cells and monocytes. Monoclonal anti-Monocyte Chemotactic Protein-1 antibody is suitable for neutralization, in which it will not neutralize the activity of recombinant human MIP-1α, recombinant mouse MIP-1α or recombinant human RANTES. Mouse anti-Monocyte Chemotactic Protein-1 antibody reacts specifically with human MCP-1/CCL2.

Specificity

The antibody will neutralize the biological activity of recombinant human MCP-1.This antibody does not exhibit cross-reactivity with recombinant human RANTES, recombinant human MIP-1α, or recombinant mouse MIP-1α..

Immunogen

recombinant human MCP-1/CCL2 expressed in E. coli.

Application

Monoclonal anti-Monocyte Chemotactic Protein-1 antibody can be used in western blotting.

Physical form

Lyophilized from a 0.2 μm solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein A.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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