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M2634 Sigma-Aldrich

Malic Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Synonym: L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
form   buffered aqueous glycerol solution
cation traces   NH4+: ≤10 μg/mg protein
foreign activity   Glutamic-Oxalacetic Transaminase ≤0.01%
  Glutamic-Pyruvic Transaminase ≤0.01%
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Mitochondrial Isozyme

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.

Biochem/physiol Actions

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Protocols

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

The continuous spectrophotometric rate determination (A340, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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