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M265 Sigma-Aldrich

Anti-Glutamate Receptor NMDAR2B (NR2B) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Glutamate Receptors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (frozen sections): 1:1,000
  immunoprecipitation (IP): 3 μL using 200 μg of rat brain
  western blot: 1 μg/mL using 35S-Protein A (with ECL dilutions may be higher)
species reactivity   rat, mouse, human
mol wt   antigen 180 kDa
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q13224
Gene Information   human ... GRIN2B(2904)
mouse ... Grin2b(14812)
rat ... Grin2b(24410)

Description

General description

The functional N-Methyl-D-Aspartate (NMDA) receptor complex consists of a NMDAR1 (NR1) subunit and atleast one NMDAR2 subunit ranging from NR2A-NR2D. These receptor complexes have been implicated in cochlear functions and neuronal plasticity. The C-terminal end of NR2B receptor may regulate synaptic localization
Anti-Glutamate Receptor NMDAR2B (NR2B) antibody is specific for NR2B subunit in mice, rats and humans. This antibody does not react with NR2A or NR2C subunits. The labeling of NR2B subunit by immunoblotting is blocked by the preadsorption of the antibody with the immunogen.

Immunogen

C-terminal portion of NR2B (amino acids 984-1104).

Application

Anti-Glutamate Receptor NMDAR2B (NR2B) antibody is suitable for use in immunohistochemistry (1:1,000 dilution, using frozen sections), immunoprecipitation (3 μL using 200 μg of rat brain), and indirect ELISA. The antibody may also be used for western blot (1 μg/mL using (35S)-Protein A) and immunoblot (180 kDa).

Physical form

Lyophilized from ammonium bicarbonate (5 mM).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

10 μg in serum bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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