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  • M266 - Anti-Glutamate Receptor NMDAR2C (NR2C) antibody produced in rabbit

M266 Sigma

Anti-Glutamate Receptor NMDAR2C (NR2C) antibody produced in rabbit

affinity isolated antibody, lyophilized powder



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Glutamate Receptors,
species reactivity   human, mouse, rat
application(s)   immunohistochemistry (frozen sections): 1:1000
  immunoprecipitation (IP): 3 μL using 200 μg of rat cerebellum.
  indirect immunofluorescence: 1:1000 using frozen sections
  western blot: 1:1000 using 200 μg cell lysate
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
mol wt   antigen mol wt 140 kDa (NR2C subunit)
  antigen mol wt 180 kDa (NR2A and NR2B bands)
storage temp.   −20°C
Gene Information   human ... GRIN2C(2905)
mouse ... Grin2c(14813)
rat ... Grin2c(24411)
biological source   rabbit
conjugate   unconjugated



N-terminal portion of NR2C (amino acids 25-130)

Physical form

Lyophilized from ammonium bicarbonate (5 mM).

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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