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M2909 Sigma-Aldrich

Murashige and Skoog Modified Basal Salt Mixture

powder, plant cell culture tested

Synonym: Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate



form   powder
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C


Formula variant

Without NH4NO3
With the macro- and micronutrients described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg’s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.


Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Safety & Documentation

Safety Information

GHS03  GHS03
Signal word 
Hazard statements 
Precautionary statements 
UN 1477 5.1 / PGII
WGK Germany 


Certificate of Analysis

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Protocols & Articles


Murashige and Skoog Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.
Keywords: Mass spectrometry, Vitamins

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