• USA Home
  • M3003 - Myokinase from rabbit muscle

EMAIL THIS PAGE TO A FRIEND
M3003 Sigma-Aldrich

Myokinase from rabbit muscle

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

Synonym: ATP: AMP phosphotransferase, Adenylate Kinase

Purchase

Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
form   ammonium sulfate suspension
mol wt   21 kDa
foreign activity   3-phosphoglyceric phosphokinase ≤0.05%
  lactic dehydrogenase and pyruvate kinase ≤0.01%
storage temp.   2-8°C

Description

General description

Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.

Application

Myokinase has been used in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate. It has also been used in a study to investigate the ligand-binding properties of its peptide fragments.

Biochem/physiol Actions

Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP

Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity.

Unit Definition

One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?