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M3058 Sigma-Aldrich

MES sodium salt

BioPerformance Certified, suitable for cell culture

Synonym: 2-(N-Morpholino)ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt

  • CAS Number 71119-23-8

  • Empirical Formula (Hill Notation) C6H12NNaO4S

  • Molecular Weight 217.22

  •  Beilstein/REAXYS Number 3765682

  •  EC Number 275-203-2

  •  MDL number MFCD00065473

  •  eCl@ss 32129211

  •  PubChem Substance ID 24896792

  •  NACRES NA.25

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffers / Buffer salts, Buffers / Buffers Salts,
Quality Level   400
grade   BioPerformance Certified
  for molecular biology
application(s)   cell culture | mammalian: suitable
impurities   endotoxin and total aerobic microbial count, tested
useful pH range   5.5 - 6.7
pKa    6.1
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, NICKase, RNase, protease, none detected
SMILES string   [Na+].[O-]S(=O)(=O)CCN1CCOCC1
InChI   1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
InChI key   IRHWMYKYLWNHTL-UHFFFAOYSA-M

Description

General description

MES is one of the so-called "Good" buffers developed for biological applications. This product is BioPerformance certified (suitable for electrophoresis, tissue culture, and molecular biology applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Application

MES sodium salt has been used as a coupling buffer during the coupling of MAbs onto amine-reactive sensors. It has also been used as a component of germination medium and shoot induction medium during in vitro plant regeneration.

Packaging

1 kg in poly bottle

25, 100, 500 g in poly bottle

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions are stable at 2-8°C for months. Sterilization should be done by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
15

References

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