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M3148 Sigma-Aldrich

2-Mercaptoethanol

for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein Registry Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 24896800

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Properties

Related Categories Antioxidants and Reducing Agents for Protein Stabilization, Biochemicals and Reagents, Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting,
grade   for electrophoresis
  for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
InChI Key   DGVVWUTYPXICAM-UHFFFAOYSA-N
product line   BioReagent
assay   99% (GC/titration)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
solubility   H2O: soluble 1 mL/mL
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture
foreign activity   DNase, RNase, protease, none detected
storage temp.   room temp

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells

Packaging

25, 100, 250, 500 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
154.4 °F
Flash Point(C) 
68 °C
Protocols & Articles

Protocols

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Protocol Guide: MTT Assay for Cell Viability and Proliferation

The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazoli...
Keywords: Antibiotics, Cancer, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

Protocol Guide: XTT Assay for Cell Viability and Proliferation

The non-radioactive, colorimetric assay system using XTT (sodium 3´-[1- (phenylaminocarbonyl)- 3,4- tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was first described by Scudiero,...
Keywords: Antibiotics, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

Peer-Reviewed Papers
15

References

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