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  • M3682 - Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal

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M3682 Sigma-Aldrich

Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal

clone ERK-PY193, purified from hybridoma cell culture

Synonym: Anti-pY-ERK

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, M1-MAR,
conjugate   unconjugated
clone   ERK-PY193, monoclonal
biological source   mouse
application(s)   capture ELISA: suitable
  immunocytochemistry: suitable
  microarray: suitable
  western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
species reactivity   rat, human
mol wt   antigen, ERK-1 44 kDa
  antigen, ERK-2 42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P28482
Gene Information   human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)

Description

General description

Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.

Specificity

The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

Immunogen

synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Application

Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:
• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• dot-blot
• immunocytochemistry
• western blotting
• immunofluorescence

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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