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M3696 Sigma-Aldrich

Anti-MAP2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Microtubule associated protein 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   flow cytometry: 1:200-1:500
  immunohistochemistry: 1:100-1:200
  western blot: 1:500-1:1,000
species reactivity   rat, human, mouse
mol wt   antigen ~280 kDa
  antigen ~72 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
Quality Level   200
concentration   ~1 mg/mL
UniProt accession no.   P11137
Gene Information   human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

Description

General description

MAP2 is a microtubule-associated protein that regulates the structure and function of the cytoskeleton. The protein also modulates morphogenesis of neurons and cell proliferation. Interactions of MAP2 with F-actin have been implicated in neurite initiation and microtubular re-organization. Decreased levels of MAP2 have been associated with transmissible spongiform encephalopathies . Anti-MAP2 antibody is specific for MAP2 in human, mice and rats.

Microtubule associated protein 2 (MAP2) is a neuron specific protein, which is highly expressed in the central nervous system. It is the member of cytoskeletal proteins. The gene is located on human chromosome 2q34.

Immunogen

synthetic peptide corresponding to residues 2-15 of human MAP2.

Application

Anti-MAP2 antibody is suitable for use in immunohistochemistry (1:500 using Zamboni’s fixative-fixed, frozen mouse spinal cord and thalamus sections) , flow cytometry (1:200-1:500), immunoblotting (approx. 72 kDa and 280 kDa) and western blot (1:500-1:1,000).

Anti-MAP2 antibody produced in rabbit has been used in immunocytochemistry.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Microtubule associated protein 2 (MAP2) plays a critical role in the formation of dendritic protrusions in astrocytes and oligodendrocytes. It maintains the homeostasis of microtubules. The protein plays an important role in neuronal plasticity.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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