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  • M3807 - Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal

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M3807 Sigma-Aldrich

Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal

clone ERK-NP2, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   ERK-NP2, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 5-25 μg/mL using rat brain extract
species reactivity   rat, human
mol wt   antigen, ERK-1 44 kDa
  antigen, ERK-2 42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P28482
Gene Information   human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)

Description

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

Specificity

The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2).

Immunogen

synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.

Application

A working concentration of 5-25 μg/mL may be used for detection by immunoblotting in rat brain extract. The antibody has also been used at working dilution of 1:50 for MAPK quantification by flow cytometry in monocyte-derived macrophages (MDM) derived from Holstein cattle.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

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Peer-Reviewed Papers
15

References

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