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M3946 Sigma-Aldrich

Anti-MNK1 antibody produced in rabbit

~1.4 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-MAPK signal-integrating serine/threonine kinase 1

  •  NACRES NA.44

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, MF-MN,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human Mnk1
species reactivity   human
mol wt   antigen ~40 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.4 mg/mL
UniProt accession no.   Q9BUB5
Gene Information   human ... MKNK1(8569)

Description

General description

MNK1 is a serine-threonine kinase that is activated by ERK or p38 MAPK signaling cascades and modulates translation through eIF4E phosphorylation. Human MNK1 has two C-terminal splice variants, namely, MNK1a and MNK1b. While MNK1a is expressed in the cytoplasm, MNK1b is mainly localized at the nucleus . Anti-MNK1 antibody is specific for human Mnk1 (40 kDa). In immunoblotting, staining of the Mnk1 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 12-27 of human MNK1, conjugated to KLH.

Application

Anti-MNK1 antibody is suitable for use in western blot (1-2 μg/mL using lysates of HEK-293T cells transfected with human Mnk1).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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WH0008569M3 Monoclonal Anti-MKNK1 antibody produced in mouse, clone S52, purified immunoglobulin, buffered aqueous solution

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