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M4065 Sigma-Aldrich

Anti-Matrix Metalloproteinase-2 antibody produced in rabbit

~1 mg/mL, IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-MMP-2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL
  western blot: 5-10 μg/mL
species reactivity   bovine, rat, mouse, human
mol wt   antigen 72 kDa (proform)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P08253
Gene Information   human ... MMP2(4313)

Description

Specificity

Recognizes MMP-2, cytoplasmic localization. Shows no cross-reactivity with the pro and active forms of other MMPs.

Immunogen

synthetic peptide from the second half of human MMP-2 (Gelatinase-A, 72 kDa Gelatinase). The immunogen is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the the first 80 amino acids.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 10 mM phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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