• USA Home
  • M4401 - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse

M4401 Sigma

Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse

clone MY-21, ascites fluid

Synonym: Myosin Light Chain Antibody - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse - Tested Applications: immunoblotting denatured and reduced myosin of muscle,indirect immunofluorescence human or chicken fibroblasts 1:200, Myosin Light Chain Antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   bovine, chicken, pig, rabbit, human
application(s)   indirect immunofluorescence: 1:200 using human or chicken fibroblasts
  western blot: suitable using denatured and reduced myosin of muscle
clone   MY-21, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 20 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... MYLK2(396356)
human ... MYLK2(85366)
biological source   mouse
conjugate   unconjugated



chicken lens membrane.

General description

Myosin is a cytoskeletal protein responsible for muscle contraction, muscle filament sliding and regulation of ATPase activity. Monoclonal anti-myosin (Light Chains 20 kDa) antibody diluted 1:200 can be used to detect MLC20. It is also useful in staining (1:10,000) 2D slab gels. Anti-myosin(Light Chains 20 kDa) antibody reacts specifically with 20 kDa light chain of chicken gizzard, chicken intestine and pig stomach myosin which is SDS, heat denatured and reduced but not with intact myosin molecule in its native state.


Monoclonal anti-myosin (Light Chains 20 kDa) antibody can be used in immunoblotting and western blotting.

Price and Availability

Antibody Explorer

Explore with Confidence

Celebrate Pi Day!
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?