• USA Home
  • M4655 - Minimum Essential Medium Eagle

EMAIL THIS PAGE TO A FRIEND
M4655 Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym: MEM

Purchase

Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   HEPES: no
NaHCO3: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
sodium pyruvate: no
phenol red: yes
storage temp.   2-8°C

Description

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’ or Earle’s salts has broadened the usefulness of this medium.

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation....
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media

Cell Culture Protocol 3: Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord...
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these ...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us...
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

MEM (Minimum Essential Medium Eagle)

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subt...
Keywords: Addition reactions, Cell culture, Culture media, Eliminations

Related Products

related product

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?