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M4819 Sigma-Aldrich

Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-H3K4me1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using whole cell extract of human HeLa cells, human Jurkat cells, or mouse NIH3T3 cells
species reactivity   bovine, Drosophila, Caenorhabditis elegans, frog, chicken, Arabidopsis thaliana, rat, human, mouse
mol wt   antigen 17 kDa
form   buffered aqueous solution
shipped in   dry ice
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P68431
Gene Information   human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)

Description

Specificity

Anti-methyl-Histone H3 [Me-Lys4] recognizes histone H3 methylated on Lys4.

Immunogen

synthetic methylated peptide corresponding to amino acid 1-8 (Me-Lys4) at the N-terminus of human histone H3, conjugated to KLH. The sequence is identical in many species.

Application

Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit detection of [Me-Lys4] histone H3 by immunoblotting, chromatin immunoprecipitation and immunoprecipitation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications.Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Histone methylation like acetylation is a complex dynamic process involved in several processes including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells. Lysine residues can be mono-, di-, and trimethylated at different heterochromatic subdomains adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N- terminal tail domains of histone H3 (Lys4, Lys9, and Lys27) are the preferred sites of methylation. Histone H3 mono-, di-, and trimethylation at Lys4 and Lys9 are carried out both in vitro and in vivo by SET domain site-specific histone methyltransferases (HMTases), including Suv39h1, Suv39h2, and G9a. Di- and trimethylation of histone H3 at Lys4 in coding regions correlates with transcriptional activity of many genes. Dimethylation of Histone H3 at Lys4 occurs at both active and inactive euchromatic regions, but not in silent heterochromatic sites, whereas trimethylation at Lys4 is present exclusively at active genes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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