M5308 Sigma-Aldrich


from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%

Synonym: (S)-2-Amino-4-(methylmercapto)butyric acid, L-2-Amino-4-(methylthio)butanoic acid

  • CAS Number 63-68-3

  • Linear Formula CH3SCH2CH2CH(NH2)CO2H

  • Molecular Weight 149.21

  •  Beilstein Registry Number 1722294

  •  EC Number 200-562-9

  •  MDL number MFCD00063097

  •  eCl@ss 32160406

  •  PubChem Substance ID 24896818




1 kg in poly bottle

10 mg in glass bottle

25, 100 g in poly bottle


L-Methionine has been used as a component of media to culture various cancer cells and to study its effects on the cell cycle. It has also been used as a component of DMEM medium to culture satellite cells from the white muscle of trouts.

Biochem/physiol Actions

Methionine is an essential amino acid which acts a precursor for transmethylation and transsulphuration. In proteins, L-methionine is the biologically active form. The adenosylation of methionine results in the formation of S-adenosyl-L-methionine (SAM), which serves as a methyl donor to various substances. L-Methionine’s metabolic product glutathione is known to regulate immune response and has antiviral action.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles


HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP™

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP™
Keywords: Chromatography, High performance liquid chromatography

Related Content

Amino Acids Reference Chart

Amino acids are the 21 standard compounds or building blocks that make up peptides and proteins, including DNA. Each amino acid is structured from an amino group and a carboxyl group bound to a tetra...
Keywords: Building blocks, Gene expression, Mass spectrometry

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?