M5308 Sigma-Aldrich


from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%

Synonym: (S)-2-Amino-4-(methylmercapto)butyric acid, L-2-Amino-4-(methylthio)butanoic acid

  • CAS Number 63-68-3

  • Linear Formula CH3SCH2CH2CH(NH2)CO2H

  • Molecular Weight 149.21

  •  Beilstein/REAXYS Number 1722294

  •  EC Number 200-562-9

  •  MDL number MFCD00063097

  •  eCl@ss 32160406

  •  PubChem Substance ID 24896818

  •  NACRES NA.26



Related Categories Allium cepa (Onion), Allium sativum (Garlic), Amino Acids, Amino Acids & Derivatives, Amino Acids & Vitamins,
Quality Level   200
biological source   non-animal source
Agency/Method   meets EP testing specifications
  meets JP testing specifications
  meets USP testing specifications
assay   99.0-101.0%
form   powder
quality   meets EP, JP, USP testing specifications
application(s)   cell culture | mammalian: suitable
  peptide synthesis: suitable
impurities   endotoxin, tested
color   white
mp   284 °C (dec.) (lit.)
solubility   H2O: 25 mg/mL
Featured Industry   Pharmaceutical (small molecule)
SMILES string   CSCC[C@H](N)C(O)=O
InChI   1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1
Gene Information   rat ... Ggt1(116568)



L-Methionine has been used as a component of media to culture various cancer cells and to study its effects on the cell cycle. It has also been used as a component of DMEM medium to culture satellite cells from the white muscle of trouts.


1 kg in poly bottle

10 mg in glass bottle

25, 100 g in poly bottle

Biochem/physiol Actions

Methionine is an essential amino acid which acts a precursor for transmethylation and transsulphuration. In proteins, L-methionine is the biologically active form. The adenosylation of methionine results in the formation of S-adenosyl-L-methionine (SAM), which serves as a methyl donor to various substances. L-Methionine’s metabolic product glutathione is known to regulate immune response and has antiviral action.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP™

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP™
Keywords: Chromatography, High performance liquid chromatography

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers


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