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M5524 Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, plant cell culture tested

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
form   powder
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg’s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity

Formulated to contain 4.3 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Other Notes

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
1

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Protocols & Articles

Protocols

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today. Below, find formulat...
Keywords: Culture media, Mass spectrometry

Murashige and Skoog Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.
Keywords: Mass spectrometry, Vitamins

Orchid Culture Media Formulations

Orchid tissue culture has its own unique requirements. Below is formulation information for a number of media that are used to successfully culture Orchidaceae.
Keywords: Mass spectrometry

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Peer-Reviewed Papers
15

References

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