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  • M5539 - Anti-Macrophage Inflammatory Protein-2 antibody produced in goat

M5539 Sigma-Aldrich

Anti-Macrophage Inflammatory Protein-2 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-MIP-2



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Chemokines, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: suitable
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P10889
Gene Information   mouse ... Cxcl2(20310)


General description

Macrophage Inflammatory Protein-2 belongs to the C-X-C family of chemokines and acts as chemoattractant for neutrophils. MIP-2 was originally identified as heparin-binding protein secreted in response to endotoxin. This chemokine acts majorly to induce influx of neutrophils following infection by pneumococci, hypersensitive reaction to viral antigen and inflammation due to glomerular nephritis. Pulmonary introduction of LPS results in rapid increase in the levels of MIP-2 producing inflammation. Anti-Macrophage Inflammatory Protein-2 antibody is specific for mouse MIP-2.


The antibody shows <5% cross-reactivity with recombinant human GROα, recombinant human GROβ and recombinant human GROγ.


recombinant mouse MIP-2 expressed in E. coli.


Anti-Macrophage Inflammatory Protein-2 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. The antibody is suitable for neutralization reactions.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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M5196 Monoclonal Anti-Macrophage Inflammatory Protein-2 antibody produced in rat, clone 40605, purified immunoglobulin, lyophilized powder
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