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M5755 Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym: MESA Buffer



Related Categories Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Running Buffers, Plant Biotechnology, Plant Molecular Biology More...
Quality Level   200
grade   for molecular biology
product line   BioReagent
form   powder
pH range   6.8 - 7.2 (reconstituted per directions)
solubility   H2O: soluble, faintly yellow
foreign activity   DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.   room temp


General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.


1 L in poly bottle


Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.


1x MESA buffer contains:
• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)


Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

TAE and TBE Running Buffers Recipe & Video

Tris acetate EDTA (TAE) and tris borate EDTA (TBE) are the two most common running buffers used in nucleic acid electrophoresis. As buffers, they have a fairly constant pH and are able to conduct ele...
Keywords: AGE, Buffers, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Polymerase chain reaction, Precipitation, Pulsed field gel electrophoresis, Sequencing

Peer-Reviewed Papers


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