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M5815 Sigma-Aldrich

Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   F12B, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
  immunoprecipitation (IP): 2 μg using 1 mg protein lysate
  indirect ELISA: suitable
  indirect immunofluorescence: suitable
  western blot: 1 μg/mL
species reactivity   mouse, rat, human
mol wt   antigen 34 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P15173
Gene Information   human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)

Description

General description

Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.

Immunogen

rat recombinant myogenin (amino acids 30-224).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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