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  • M5815 - Monoclonal Anti-Myogenin antibody produced in mouse

M5815 Sigma

Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Gene Regulation,
species reactivity   mouse, rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
  immunoprecipitation (IP): 2 μg using 1 mg protein lysate
  indirect ELISA: suitable
  indirect immunofluorescence: suitable
  western blot: 1 μg/mL
clone   F12B, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 34 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)
biological source   mouse
conjugate   unconjugated



rat recombinant myogenin (amino acids 30-224).

General description

Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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