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  • M5917 - Anti-Macrophage Inflammatory Protein-1β antibody produced in goat

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M5917 Sigma-Aldrich

Anti-Macrophage Inflammatory Protein-1β antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-MIP-1β

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Description

General description

Macrophage Inflammatory Protein-1-β is a member of the chemokine family and is produced in macrophages when stimulated by bacterial endotoxins. Anti-macrophage inflammatory protein-1β antibody can be used in immunoblotting. Goat anti-macrophage inflammatory protein-1β antibody reacts specifically with recombinant mouse MIP-1 β but does not react with recombinant human MIP-1 β or recombinant mouse or human MIP-1α.

Macrophage inflammatory protein-1 β (CCL4) belongs to a family of MIP-1 chemotactic cytokines (CC). The other members are CCL3 (MIP-1α), CCL9/10 (MIP-1δ) and CCL15 (MIP-1γ). The members of MIP-1 family have the ability to self-associate and form aggregates of high molecular weight. The self- aggregation is a reversible process and occurs in T cells, B cells and macrophages in response to inflammatory factors and bacterial endotoxins. This response results in inflammation and platelet degranulation
Anti-Macrophage Inflammatory Protein-1β antibody is specific for mouse MIP-1 β. It shows less than 2% cross reactivity with recombinant mouse MIP-1α, recombinant human MIP-1α and MIP-1β.

Immunogen

recombinant mouse MIP-1β expressed in E. coli.

Application

Anti-Macrophage Inflammatory Protein-1β antibody may be for immunoblotting at a working concentration of 1.0 μg/ml. The antibody is suitable for neutralization reactions.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, containing 5% trehalose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein G.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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M6792 Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, IgG fraction of antiserum, lyophilized powder

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