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  • M6053 - Anti-Matrix Metalloproteinase-3, Propeptide Region antibody produced in rabbit

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M6053 Sigma-Aldrich

Anti-Matrix Metalloproteinase-3, Propeptide Region antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-MMP-3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1000
species reactivity   human
mol wt   antigen 59 kDa
  antigen (pro-form) 57 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P08254
Gene Information   human ... MMP3(4314)

Description

General description

Matrix metalloproteinase-3 (MMP-3) is also known as stromelysin 1 and the gene encoding it is localized on human chromosome 11q22.3.

Specificity

Reacts with reduced and to a lesser extent non-reduced MMP-3. The propeptide is shed after activation; therefore, the antibody recognizes the pro-form of MMP-3 and not the active forms. May be used to distinguish the latent from the active form of MMP-3. Recognizes Stromelysin-1 and Stromelysin-2, but not Stromelysin-3 or other MMP family members.

Immunogen

synthetic peptide corresponding to the propeptide region of human matrix metalloproteinase-3 (stromelysin-1).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide.

Biochem/physiol Actions

Matrix metalloproteinase-3 (MMP-3) plays an important role in cartilage degradation. It can act on a large number of substrates like type II, III, IV, IX and X collagens, laminin and fibronectin. MMP-3 can also activate other matrix metalloproteinases like MMP-1,-2 and -13. It has been associated with rheumatoid arthritis.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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