• USA Home
  • M6073 - Anti-MIB1 (N-terminal) antibody produced in rabbit

M6073 Sigma

Anti-MIB1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-DAPK-interacting protein 1, Anti-DIP-1, Anti-KIAA1323, Anti-Mindbomb homolog 1, Anti-Ubiquitin ligase mind bomb



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to E1,E2,E3 Ligase, Antibodies to Protein Degradation,
species reactivity   human
application(s)   western blot: 1:125-1:250 using lysates of HEK-293T cells over expressing MIB1
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MIB1(57534)
mouse ... Mib1(225164)
rat ... Mib1(307594)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 117-134 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

General description

Mindbomb homolog 1 (MIB1) is an ubiquitin ligase which possesses two substrate recognition domains at the carboxy terminal, ankyrin repeats and an amino terminal zinc finger domain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Mindbomb homolog 1 (MIB1) aids in the ubiquitination and internalization of Notch ligands such as the protein Delta. It also acts as a positive regulator in the activation of nuclear factor-κB (NF-κB).

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

WH0023405M1 Monoclonal Anti-DICER1 antibody produced in mouse, clone 2F12, purified immunoglobulin, buffered aqueous solution
WH0057534M3 Monoclonal Anti-MIB1 antibody produced in mouse, clone 2A9, purified immunoglobulin, buffered aqueous solution
HPA019100 Anti-MIB1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
M5948 Anti-MIB1 (C-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?