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  • M6190 - Monoclonal Anti-MYOD1 antibody produced in mouse

M6190 Sigma

Monoclonal Anti-MYOD1 antibody produced in mouse

clone 5.2F, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Myogenic Differentiation Antigen 1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Gene Regulation and Expression,
species reactivity   human, chicken, mouse, rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL
  immunohistochemistry (frozen sections): 2-4 μg/mL
  immunoprecipitation (IP): 2 μg using 1 mg protein lysate
  western blot: 1 μg/mL (reacts with the ~45 kDa protein)
clone   5.2F, monoclonal
concentration   1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 45 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MYOD1(4654)
mouse ... Myod1(17927)
rat ... Myod1(337868)
biological source   mouse
conjugate   unconjugated



recombinant mouse MyoD1 protein.

General description

Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Biochem/physiol Actions

Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest.

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WGK Germany  nwg
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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