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M6542 Sigma-Aldrich

Macrophage Inflammatory Protein-1β from mouse

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: MIP-1β, mMIP-1β

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Properties

Related Categories Cell Biology, Cell Culture, Chemokines, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Cytokines, Growth Factors, Chemokines for Stem Cell Biology,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE and N-terminal analysis)
form   lyophilized powder
potency   40-100 ng/mL ED50/EC50
mol wt   mol wt 7.8  kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
suitability   suitable for cell culture
UniProt accession no.   P14097
storage temp.   −20°C
Gene Information   mouse ... Ccl4(20303)

Description

Analysis Note

The biological activity of MIP-1β was tested in culture by measuring its ability to inhibit hematopoietic stem cell proliferation in an in vitro colony assay.

Biochem/physiol Actions

MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. MIP-1 can synergize with the hematopoietic growth factors granulocytemacrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin

General description

MIP-1α and MIP-1β (macrophage inflammatory protein) were originally co-purified from LPS stimulated mouse macrophages. Recombinant, mouse MIP-1β (rmMIP-1β) consists of 69 amino acids. MIP- 1β belongs to the chemokine β subfamily which is characterized by a C-C configuration at the first two cysteines. MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. Although other cytokines, such as IL-1alpha, IL-1β and TNF have endogenous pyrogenic activity, the pyrogenic effects of these cytokines can be inhibited by cyclooxgenase blockers, while the pyrogenicity of MIP- 1β is unaffected by these agents. MIP-1β can synergize with the hematopoietic growth factors granulocyte macrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis


Cell Culture Guide

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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