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  • M6552 - Monoclonal Anti-Matrix Metalloproteinase-3 antibody produced in mouse

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M6552 Sigma-Aldrich

Monoclonal Anti-Matrix Metalloproteinase-3 antibody produced in mouse

clone SL-1 IIIC4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-MMP-3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix/Cytoskeleton,
conjugate   unconjugated
clone   SL-1 IIIC4, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using heat/pressure cooker pre-treatment
  immunohistochemistry (frozen sections): 1:50-1:100
  immunoprecipitation (IP): 10 using protein lysate (μL/mg)
  indirect immunofluorescence: 4-5 μg/mL
  western blot: 1:200-1:400 using conditioned medium from PMA-treated HT-1080 cells or bladder, breast, or ovarian carcinoma
species reactivity   human
mol wt   antigen ~48 kDa (active form)
  antigen ~57 kDa (latent form)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P08254
Gene Information   human ... MMP3(4314)

Description

Immunogen

APMA-activated human matrix metalloproteinase-3 (stromelysin-1).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in 10 mM phosphate buffered saline and 0.2% bovine serum albumin, pH 7.4, containing 0.1% sodium azide.

Safety & Documentation

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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HPA007875 Anti-MMP3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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