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M6667 Sigma-Aldrich

Monocyte Chemotactic Protein-1 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, cell culture tested

Synonym: MCP-1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Chemokines, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones,
biological source   human
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE and N-terminal analysis)
form   lyophilized powder
potency   5-60 ng/mL EC50
mol wt   mol wt 8.7 kDa
packaging   pkg of 10 μg
impurities   endotoxin, tested
suitability   cell culture tested
UniProt accession no.   P13500
storage temp.   −20°C
Gene Information   human ... CCL2(6347)

Description

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Application

Monocyte chemotactic protein-1 (MCP-1) has been used to study its effects on the motility of lymphoblastoid cell line NC–NC cells.

Biochem/physiol Actions

The biological activity is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED<SUB>50</SUB> for this effect is typically 5-30 ng/mL.

Measured by its ability to chemoattract 2-day cultured human monocytes, Matsushima, K., et al. (1989) J. Exp. Med. 169:1485, the ED<SUB>50</SUB> for this effect is typically 5-20 ng/mL.

Monocyte chemoattractant protein-1 (MCP-1) triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. MCP-1 chemoattracts monocytes, macrophages, T cells and natural killer cells. The protein is overexpressed in atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.

General description

Monocyte chemoattractant protein-1 (MCP-1) or monocyte chemotactic and activating factor (MCAF) is part of the C-C chemokine family. The gene encoding this protein is localized on human chromosome 17q12.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Peer-Reviewed Papers
15

References

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