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M6899 Sigma-Aldrich

Murashige and Skoog Basal Salts with minimal organics

powder, suitable for plant cell culture

Synonym: MSMO

  •  NACRES NA.72

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
Quality Level   200
form   powder
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Murashige and Skoog Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.
Keywords: Mass spectrometry, Vitamins

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Peer-Reviewed Papers
15

References

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