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M6908 Sigma-Aldrich

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

Synonym: MPO, Peroxidase, myelo

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Description

General description

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24. The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.

Application

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.

Myeloperoxidase from human leukocytes has been used:
• as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue
• as a neutrophil extracelullar trap (NET) component to stimulate 106 dendritic cells or 106 macrophages
• as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO

Biochem/physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells. MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease. MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H2O2).

Other Notes

Lysosomal heme-containing protein.

Unit Definition

One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of 1/4 micromole of tetraguaiacol per minute at pH 7.0 and 25 °C

Physical form

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

Analysis Note

Source material tested and found negative for antibody to HIV and for HBSAG.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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