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M7070 Sigma

Anti-MO25 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CAB39, Anti-CGI-66, Anti-Calcium binding protein 39

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   rat, mouse, human
application(s)   western blot: 0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates
clone   polyclonal
concentration   ~2 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~40 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAB39(51719)
mouse ... Cab39(12283)
rat ... Cab39(301574)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 308-326 located near the C-terminus of human MO25. This sequence is identical in mouse, rat, dog, and bovine MO25.

General description

MO25 is a 40 kDa scaffolding protein that interacts with STRADα and LKB1 to regulate tumor suppression. MO25 activates LKB1 and also localizes LKB1 in the cytoplasm . Anti-MO25 antibody is specific for MO25 in humans, rats and mice. In immunoblotting, staining of the MO25 band is specifically inhibited by the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Application

Anti-MO25 antibody is suitable for use in western blot (0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates).

Price and Availability

Safety & Documentation

Safety Information

WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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M7195 Anti-MO25 (C-terminus) antibody produced in rabbit, ~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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