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  • M7317 - Monoclonal Anti-MDR3 P-Glycoprotein antibody produced in mouse

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M7317 Sigma-Aldrich

Monoclonal Anti-MDR3 P-Glycoprotein antibody produced in mouse

250 μg/mL, clone P3II-26, tissue culture supernatant

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Multi-Drug Resistance, Antibodies to Drug Efflux Pumps,
conjugate   unconjugated
clone   P3II-26, monoclonal
biological source   mouse
application(s)   immunocytochemistry: 1:20-1:50 using acetone-fixed cytospin preparations
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (not suitable for human tissues)
  immunohistochemistry (frozen sections): 1:20 using acetone-fixed sections
  western blot: suitable
species reactivity   human
shipped in   dry ice
storage temp.   −20°C
antibody form   tissue culture supernatant
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
concentration   250 μg/mL
UniProt accession no.   P21439
Gene Information   human ... ABCB11(8647), ABCB4(5244)

Description

General description

Multidrug resistance protein 3 (MDR3) is also known as ATP binding cassette subfamily B member 4. It is expressed in hepatocytes and made up of 1279 amino acids. The gene encoding it is localized on human chromosome 7q21.12 and consists of 27 exons.

Specificity

Reacts with an internal epitope of MDR3. Does not cross-react with human MDR1 P-gp.

Immunogen

MDR3 P-gp (amino acids 629-692) GST fusion protein.

Physical form

Supplied in serum-free medium containing 0.7% bovine serum albumin and 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Multidrug resistance protein 3 (MDR3) acts as an ATP-dependent exporter and transfers phospholipids, particularly phosphatidylcholine (PC), into bile. Dysfunctioning of MDR3 leads to excess of bile salts in primary bile and further leads to liver diseases.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

ABC Transporters and Cancer Drug Resistance

Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a group of...
BioFiles 2007, 2.4, 7.
Keywords: Absorption, Cancer, Clinical, Cloning, Combinatorial synthesis, Diseases, Enzymology, Gene expression, Genetic, Immunology, Pharmaceutical, Reductions, Transfection

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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