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M7556 Sigma-Aldrich

Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

Synonym: Anti-ERK-2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5 μg using 0.5-1 mg of a mouse 3T3 fibroblast lysate
  western blot: 0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells
species reactivity   mouse, human, rat
mol wt   antigen 42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P28482
Gene Information   human ... MAPK1(5594)
mouse ... Ephb2(13844), Mapk1(26413)
rat ... Mapk1(116590)

Description

General description

Mitogen-activated protein kinases (MAPKs) regulate a wide range of functions by modulating cellular signal transduction. These functions include cellular growth, differentiation, movement and apoptosis. Conventional MAPKs comprise of the ERK1/2, p38, JNK and ERK5 kinase families. ERK1 and ERK2 are highly conserved serine/threonine kinases that are responsive to growth factors
Anti-MAP Kinase 2 (ERK-2) antibody is specific for MAPK2 (42 kDa) in mice, humans and rats. This antibody does not cross react with MAP kinase 1 (ERK-1) nor does it interfere with the kinase activity.

Immunogen

recombinant mouse MAP Kinase 2 (MAPK2, ERK-2).

Application

Anti-MAP Kinase 2 (ERK-2) antibody is suitable for use in western blot (0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells). The product can also be used for immunoprecipitation (5 μg, using 0.5-1 mg of a mouse 3T3 fibroblast lysate).

Physical form

Solution in 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and ≤ 0.1% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
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References

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