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M8042 Sigma

Minimum Essential Medium Eagle

Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: MEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   NaHCO3: yes
L-glutamine: no
phenol red: yes
HEPES: no
sodium pyruvate: yes
storage temp.   2-8°C

Description

General description

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. See formulation for details. Often used in cytogenetic analysis when supplemented with FBS.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.292 g/L L-glutamine

Application

Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook1

1 EMD Millipore Corporation, Stem Cell Bioprocessing Group, 80 Ashby Rd, Bedford, MA 01730, USA.
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

Related Content

Minimum Essential Medium Eagle - MEM Media

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain sub...
Keywords: Addition reactions, Adhesion, Cell culture, Cloning, Culture media, Eliminations, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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