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M8042 Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: MEM

  •  NACRES NA.75

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   glucose: yes
stable glutamine: no
phenol red: yes
Earle’s salts (5% CO2): yes
sodium pyruvate: yes
NaHCO3: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
shipped in   ambient
storage temp.   2-8°C

Description

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Application

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.

Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.292 g/L L-glutamine

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Single- & multi-channel pipettes from BRAND

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Protocols & Articles

Articles

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook
Stem Cell Bioprocessing Group
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

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MEM (Minimum Essential Medium Eagle)

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subt...
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Peer-Reviewed Papers
15

References

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