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M8318 Sigma-Aldrich

Anti-AtMPK3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Arabidopsis thaliana MAP Kinase 3

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   Arabidopsis thaliana
application(s)   western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves
  western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 43 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   Arabidopsis thaliana ... ATMPK3(823706)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 359-370 at the C-terminus of Arabidopsis thaliana MPK3. This sequence is specific to AtMPK3 (not found in the other 20 known AtMPK isoforms). It has limited homology (58% identity) to the AtMPK6 isoform and shares significant homology (67% identity, continuous epitope) with the Nicotiana tabacum ortholog WIPK.

General description

AtMPK3 is a component of the plant MAPK signaling cascade that functions downstream of the FLS2 receptor and regulates the innate response to bacterial and fungal infections. AtMPK3 is also involved in the phosphorylation of VIP1 in response to Agrobacterium infection and the subsequent transfer of the bacterial T-DNA into the nucleus. RNAi mediated inhibition of AtMPK6 causes prolonged activation of AtMPK3 . Anti-AtMPK3 antibody is specific for AtMPK3 (43 kDa). The product also reacts with Nicotiana tabacum and p56 in Papaver rhoeas . In immunoblotting, staining of AtMPK3 is specifically inhibited with AtMPK3 immunizing peptide (Arabidopsis thaliana, amino acids 359-370).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-AtMPK3 recognizes AtMPK3.

Application

Anti-AtMPK3 antibody is suitable for use in western blot (1:1000 using pollen extracts of Papaver rhoeas)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mitogen-activated protein kinases (MAPKs) regulate a variety of cellular activities ranging from gene expression, cell proliferation, motility, metabolism and apoptosis. Activation of MAPKs requires phosphorylation on threonine and tyrosine residues in the kinase regulatory domain by upstream dual specificity kinases, termed MAPK kinases (MAPKKs/MEK). MAPKKs, in turn are activated through phosphorylation by a highly diverse group of MAPKK kinases (MAPKKK/MEKK). In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4 and AtMPK6. All the three MAPKs have been linked to diverse stress responses, including oxidative stress signaling, pathogen defence, drought, high salt, cold and touch. Orthologs of AtMPK3 and AtMPK6 in tobacco, wound-inducible protein kinase (WIPK) and salicylate-inducible protein kinase (SIPK) respectively, are also activated by both biotic and abiotic stresses. Arabidopsis MAPKs, AtMPK3, AtMPK4 and AtMPK6 are rapidly and transiently activated by stress responses, by activation of distinct signal transduction pathways, involving upstream MAPKKKs (e.g. AtANP1 and AtMEKK1) and MAPKKs including AtMKK1/2 and AtMKK4/5.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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