M8407 Sigma-Aldrich

Methotrexate hydrate

powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

Synonym: 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate

  • CAS Number 133073-73-1

  • Empirical Formula (Hill Notation) C20H22N8O5 · xH2O

  • Molecular Weight 454.44 (anhydrous basis)

  •  MDL number MFCD00150847

  •  PubChem Substance ID 24897252



Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Companion Products and Reagents,
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
solubility   H2O: insoluble
suitability   suitable for cell culture
Mode of action   enzyme | inhibits
antibiotic activity spectrum   neoplastics
storage temp.   −20°C



Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Recommended for use in cell culture and molecular biology applications at 0.01-300 μM in nucleoside-free cell culture medium to select for DHFR expression.

Biochem/physiol Actions

Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

Safety & Documentation

Safety Information


Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I make a solution of Methotrexate hydrate, Product M8407?
Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.
How do I store a stock solution of Methotrexate hydrate, Product M8407?
A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.
How do I sterilize a solution of Methotrexate hydrate, Product M8407?
A solution of methotrexate can be sterile filtered using a 0.22 mm filter
How often doesMethotrexate hydrate, Product M8407, need to be added to the media during selection?
Fresh methotrexate should be added each time the culture media is changed.Regardless of cell growth, most media will need to be changed at least once a week.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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