M8410 Sigma-Aldrich

Mineral oil

light oil (neat), BioReagent, suitable for mouse embryo cell culture



Related Categories Cell Culture, Mouse Embryo Culture, Reagents and Media Supplements, Specialty Media Systems
sterility   non-sterile
product line   BioReagent
form   light oil (neat)
storage condition   protect from light
refractive index   n20/D 1.467 (lit.)
density   0.84 g/mL at 25 °C (lit.)
suitability   suitable for mouse embryo cell culture



1 L in glass bottle

100, 500 mL in glass bottle

5 mL in poly bottle


Recommended for overlaying mouse embryo cultures to prevent drying.


This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
>233.6 °F
Flash Point(C) 
>112 °C


Certificate of Analysis


Certificate of Origin


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M5310 Mineral oil, light oil ((neat)), BioXtra, suitable for mouse embryo cell culture

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