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  • M8413 - Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody produced in mouse

M8413 Sigma-Aldrich

Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody produced in mouse

clone 35509.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-MCP-2



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   35509.11, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: suitable
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P80075
Gene Information   human ... CCL8(6355)


General description

Monocyte Chemotactic Protein-2 (MCP-2) belongs to a family of C-C chemotactic cytokines. MCP-1, -3, -4, and -5 are the other five monocyte chemo attractants identified. The MCP cytokines are involved in recruitment of leukocytes to the sites of inflammation, and activate NK cells and CD4+ and CD8+ T lymphocytes. Additionally, MCP cytokines can activate basophils to secrete histamines and also attract eosinophils. MCPs play an important role in pathological conditions such as systemic sclerosis, myocardia and cardiac ischemia. Anti-monocyte chemotactic protein 2 antibody specifically reacts with human MCP-2. The antibody shows <20% cross reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.


Neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.


recombinant human MCP-2 expressed in E. coli.


Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody may be used in ELISA at a concentration of 2.0 μg/ml. For immunoblotting a working concentration of 1-2 μg/ml may be used. The antibody is suitable for neutralization reactions; ND50 is 1-4 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein A.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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