• USA Home
  • M8645 - Anti-Mouse IgG (whole molecule) antibody produced in goat

M8645 Sigma

Anti-Mouse IgG (whole molecule) antibody produced in goat

affinity isolated antibody, lyophilized powder



Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   lyophilized powder
application(s)   Ouchterlony double diffusion: suitable
conjugate   unconjugated
storage temp.   2-8°C


Other Notes

Antibody adsorbed with human serum proteins


IgG purified from pooled normal mouse serum.


Reconstitute with 1 mL of 0.135 M sodium chloride.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2


Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays (50 μgram) .

General description

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?