• USA Home
  • M8787 - Magnesium chloride solution

EMAIL THIS PAGE TO A FRIEND
M8787 Sigma-Aldrich

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Purchase

Properties

Related Categories Buffers / Buffer Salts, Core Bioreagents, General Reagents, Life Science Reagents for PCR, Molecular Biology,
Quality Level   300
grade   PCR Reagent
form   liquid
packaging   vial of 1.5 mL
concentration   25 mM±1 mM
color   colorless
Featured Industry   Agriculture
Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, none detected
storage temp.   2-8°C
SMILES string   Cl[Mg]Cl
InChI   1S/2ClH.Mg/h2*1H;/q;;+2/p-2
InChI key   TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Description

Application

• For routine PCR amplifications.
• Optimization of MgCl2 concentration in PCR reactions for improved yield.

Suitability

Suitable for optimization of polymerase chain reactions.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Qualitative Multiplex PCR Assay for Assessing DNA Quality from FFPE Tissues and Sources of Damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is ...
Steve Michalik, Christopher Williams
LSI Edition 23
Keywords: AGE, Amplification, Cancer, Cell disruption, Clinical, Degradations, Forensic, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Whole genome amplification

Protocols

Assessing DNA Quality from FFPE Tissues and Other Sources of Damaged DNA with Qualitative Multiplex PCR Assay

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is ...
Keywords: AGE, Cell disruption, Degradations, Forensic, Polymerase chain reaction, Polymerase chain reaction - quantitative

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Keywords: AGE, Amplification, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Poisons, Polymerase chain reaction, Polymerase chain reaction - quantitative

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?