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M8823 Millipore

Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

Synonym: Anti-ddddk, Anti-dykddddk, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, FLAG Affinity Gels, FLAG System,
conjugate   magnetic beads
clone   M2, monoclonal
application(s)   affinity chromatography: suitable
  immunoprecipitation (IP): suitable
form   (Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).)
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
shelf life   2 yr at -20 °C
analyte chemical class(es)   proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)
bead size   20-75 μm
matrix   superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
capacity   ≥0.6 mg/mL binding capacity

Description

General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Application

Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Browse additional application references in our FLAG® Literature portal.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

1 mL in poly bottle

Features and Benefits

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

Other Notes

Do not use magnetic stirring system as this will destroy the resin beads.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Anti-FLAG® M2 Magnetic Beads

Anti-FLAG® M2 magnetic beads provide an easy, fast and convenient method for the detection and capture of fusion proteins with the FLAG® peptide sequence. Beads are composed of a murine derived, anti...

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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