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M8945 Sigma-Aldrich

Matrix Metalloproteinase-9 human

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous solution

Synonym: Gelantinase-B, Gelatinase, 95 kDa, MMP-9

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Description

Biochem/physiol Actions

MMP-9 degrades the collagens that make up the extracellular matrix, which has a role in the control of angiogenesis.

Physical form

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 10 mM calcium chloride, 150 mM sodium chloride, and 0.05% Brij 35.

Analysis Note

The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the molecular weight of product M8945 (Matrix Metalloproteinase-9 Human)
Human MMP-9 consists of a 688 amino acid residue recombinant protein which has a predicted molecular mass of approximately 77 kDa. By SDS-PAGE the apparent molecular mass is approximately 93 kDa.
Does product M8945 (Matrix Metalloproteinase-9 Human) need to be activated?
To activate recombinant human MMP-9, prepare a p-aminophenylmercuric acetate (APMA) solution in DMSO. Add the AMPA solution to the MMP-9 to a final APMA concentration of 1 mM. Incubate at 37 deg. C. for 16 - 24 hours.
Is product M8945 (Marix Metalloproteinase-9 Human) endotoxin free?
This product contains < 1.0 EU/ug of the enzyme.
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