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M9015 Sigma-Aldrich

Malachite Green oxalate salt

certified by the Biological Stain Commission

Synonym: N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4

  • CAS Number 2437-29-8

  • Empirical Formula (Hill Notation) C23H25N2 · C2HO4 · 0.5C2H2O4

  • Molecular Weight 463.50

  •  Colour Index Number 42000

  •  Beilstein/REAXYS Number 3644550

  •  EC Number 219-441-7

  •  PubChem Substance ID 24897308

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Hematology and Histology, M, Stains & Dyes, A to Z,
Quality Level   200
grade   certified by the Biological Stain Commission
form   powder or crystals
composition   Dye content, ≥90%
application(s)   hematology: suitable
  histology: suitable
mp   144-150 °C
solubility   H2O: 1 mg/mL, blue to very deep blue
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
InChI   1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
InChI key   REZFCYOVFCJTDG-UHFFFAOYSA-M

Description

General description

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.

Application

Malachite Green oxalate salt has been used for phosphate quantification.

Malachite Green oxalate salt has been used:
• in the preparation of Alexander staining solution for cell viability test
• in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
• as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method

Packaging

25, 100 g in glass bottle

Suitability

Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton.

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN2811 - class 6.1 - PG 3 - EHS - Toxic solids, organic, n.o.s., HI: all
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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