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M9067 Sigma-Aldrich

Monoclonal Anti-MRP1 antibody produced in mouse

~2 mg/mL, clone QCRL-1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Multidrug Resistance Associated Protein 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Multi-Drug Resistance, Antibodies to Drug Efflux Pumps,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: 10-20 μg/mL using cultured HeLa cells or HepG2 human hepatocellular carcinoma cells
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
clone   QCRL-1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~190 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ABCC1(4363)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   P33527

Description

Immunogen

non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within amino acids 918-924 of human MRP1.

General description

Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

Does not cross-react with the human MDR1 and MDR3, mouse MRP1, and human MRP2, MRP3, MRP4, MRP5, and MRP6 gene products. The antibody may cross-react with canine MRP1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

200 μL in glass insert

Biochem/physiol Actions

Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance to chemotherapy to cells in solid tumors.

Safety & Documentation

Safety Information

WGK Germany  -
Protocols & Articles

Articles

ABC Transporters and Cancer Drug Resistance

Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a group of...
BioFiles 2007, 2.4, 7.
Keywords: Absorption, Cancer, Clinical, Cloning, Combinatorial synthesis, Diseases, Enzymology, Gene expression, Genetic, Immunology, Pharmaceutical, Reductions, Transfection

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Therapeutic uses, Western blot

Peer-Reviewed Papers
15

References

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