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M9130 Sigma-Aldrich

4-Methylumbelliferyl-β-D-glucuronide hydrate

≥98% (HPLC), BioReagent, for identification of transformed plants

Synonym: 4-Methylumbelliferyl-β-D-glucopyranosiduronic acid, MUG

  • CAS Number 881005-91-0

  • Empirical Formula (Hill Notation) C16H16O9 · xH2O

  • Molecular Weight 352.29 (anhydrous basis)

  •  MDL number MFCD09039280

  •  PubChem Substance ID 329818083

  •  NACRES NA.52

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Properties

Related Categories Biochemicals and Reagents, Cloning, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,
Quality Level   200
grade   for molecular biology
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
solubility   pyridine: 50 mg/mL, clear, colorless to very faintly yellow
storage temp.   −20°C
SMILES string   O.CC1=CC(=O)Oc2cc(O[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)C(O)=O)ccc12
InChI   1S/C16H16O9.H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22;/h2-5,11-14,16,18-20H,1H3,(H,21,22);1H2/t11-,12-,13+,14-,16+;/m0./s1
InChI key   URVSQZMOFUEQAW-YYHOVTOASA-N

Description

General description

MUG is a chemical commonly used for the detection of transformed plants. MUG shows low levels of fluorescence until treatment with glucuronidase (GUS) encoded in transformed plants. Transformed plants can be identified by their MUG fluorescence.

Application

Suitable for fluorescent detection of glucuronidase gene expression in plants.

Packaging

1 g in poly bottle

25, 100, 250 mg in poly bottle

Reconstitution

Product is soluble in molecular biology grade water at a concentration of 0.35 mg/ml.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

4-Methylumbelliferyl β-D-glucuronide hydrate

β-Glucuronidase (GUS) from E. coli has become the reporter enzyme of choice for genetic plant research. 4-Methylumbelliferyl ®-D-glucuronide (MUG) is commonly used as a substrate for detecting GUS ge...
Keywords: Gene expression, Genetic, Melting

Fermentation: Enzymes, Substrates and SCFA

The influence of probiotics on the metabolic activity of intestinal microbiota may be evaulated by detecting bacterial enzymatic activity in fecal samples, specifically β-glucosidase, β-glucuronidase...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Absorption, Cancer, Carcinogens, Cell proliferation, Chromatography, Enzyme activity, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Metabolites, Prebiotics, Probiotics, Thin layer chromatography

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Cancer, Carcinogens, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Molecular biology, Nucleic acid hybridization, PAGE, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Peer-Reviewed Papers
15

References

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