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M9170 Sigma-Aldrich

Macrophage Colony-Stimulating Factor from mouse

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: CSF-1, M-CSF, mCSF-1, mM-CSF

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones,
Quality Level   200
biological source   mouse
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
potency   0.500-3.000 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 18.2 kDa (containing 156 amino acid residues)
packaging   pkg of 10 μg
application(s)   cell culture | mammalian: suitable
impurities   ≤1.000 EU/μg
color   white
UniProt accession no.   P07141
storage temp.   −20°C
Gene Information   mouse ... Csf1(12977)

Description

Application

Macrophage Colony-Stimulating Factor from mouse has been used:
• in the initiation of osteoclastogenesis in bone marrow monocyte cells
• to stimulate osteoclast differentiation in non-adherent hematopoietic cell
• for the generation of blood-derived stem cells

Biochem/physiol Actions

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

Physical form

Lyophilized from a sterile filtered buffered aqueous solution.

Analysis Note

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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