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M9274 Sigma-Aldrich

Murashige and Skoog Basal Medium

plant cell culture tested, with sucrose and agar

Synonym: MS Basal Medium

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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Protocols & Articles

Protocols

Murashige and Skoog Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.
Keywords: Mass spectrometry, Vitamins

Peer-Reviewed Papers
15

References

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