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M9570 Sigma-Aldrich

Anti-Matrix Metalloproteinase-9 antibody produced in goat

affinity isolated antibody

Synonym: Anti-MMP-9, MMP-9 Antibody - Anti-Matrix Metalloproteinase-9 antibody produced in goat, Mmp-9 Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Matrix Metalloproteinases and TIMPs,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry: 5-15 μg/mL using cells or tissues
  immunoprecipitation (IP): 25 μg/mL using conditioned media of transfected NSO cells
  western blot: 0.1 μg/mL
species reactivity   mouse
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P41245
Gene Information   mouse ... Mmp9(17395)

Description

General description

Matrix metalloproteinase-9 (MMP-9) is a pro-inflammatory, zinc-dependent proteinase which is also known as 92kDa gelatinase. It is produced by various cells like granulocytes and mononuclear cells. The gene encoding it is localized on human chromosome 20q11.2-q13.1.

Specificity

This antibody recognizes pro and active mouse MMP-9. Immunoblotting and ELISA applications show an ~10% cross-reactivity with recombinant human MMP-9.

Immunogen

purified, NSO-derived, recombinant mouse matrix metalloproteinase-9.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Target description

Matrix Metalloproteinase-9 encodes an enzyme that degrades type IV and V collagens.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Matrix metalloproteinase-9 (MMP-9) has been studied as a biomarker of nervous tissue inflammation in multiple sclerosis (MS).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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